Salmon Sperm Carrier Dna

Erwin Chargaff - Wikipedia
Erwin Chargaff (11 August 1905 – 20 June 2002) was an Austro-Hungarian biochemist who immigrated to the United States during the Nazi era and was a professor of biochemistry at Columbia University medical school. ... Read Article

Enzymatic Synthesis Of Deoxyribonucleic Acid
628 Enzymatic Synthesis of DNA. XXV Vol. 243, No. 3 Salmon sperm and calf thymus DNAs were isolated by the method of Kay, Simmons, and Dounce (13); partial pancreatic digestion of this DNA was carried out by the procedure of ... Fetch Document

Quantitative Multiplex Methylation-Specific PCR Assay For The ...
Containing 40 g/ml proteinase K and 200 ng of salmon sperm carrier DNA. After proteinase K treatment, samples were heat-inactivated at 70°C for 10 min and centrifuged at 16000 g for 10 min. Fifty l of the supernatant were used ... Document Viewer

Cell, Vol. 47, 777-784, December 5, 1966, Copyright 0 1966 By ...
The presence of poly(dl-dC) . poly(dl-dC) as carrier DNA. Other experiments with sheared salmon sperm DNA as carrier gave similar results, but with a lower background of nonspecific binding (e.g., Figure 3). The analysis in Fig- ure 2 also used increasing amounts of specific (pF2) or ... Retrieve Full Source

No. Of 15, 262, OF CHEMISTRY ~..s.A. 4x174 Formation Of ...
0.1 ml of 1 mg/mf heat-denatured salmon sperm DNA (as carrier), and 4 ml of 5% trichloroacetic acid. After 10 min on ice, acid-insoluble material was collected on glass fibers filters (Enzo), which were then washed with 1% trichloroacetic acid and 95% ethanol and dried under a heat lamp. The radioactivity retained was then determined. Reac- ... View Full Source

01 LabRef Sect.1 P.1 42 - Cold Spring Harbor Lab Press
Salmon sperm DNA (5–10 kb) is preferred for use as a carrier. Small-scale Preparation Dissolve 10 mg of salmon sperm DNA in 1 ml of sterile H 2O in a polycar-bonate tube. Sonicate five times, 30 seconds each time, at maximum power, chilling tube on ice between bursts. Check molecular size of DNA by gel elec- ... Visit Document

Quick And Easy Yeast Transformation Using The LiAc/SS carrier ...
Single-stranded carrier DNA (2.0 mg ml)1 Dissolve 200 mg of salmon sperm DNA in 100 ml of sterile TE (10 mM Tris–HCl, 1 mM Na 2EDTA, pH 8.0) using a magnetic stir plate at 4 1C. This should take ... Retrieve Document

Recombinational Circularization Of Salmonella Phage P22 DNA
Recombinational Circularization of Salmonella Phage P22 DNA STEVEN WEAVER’ AND MYRON LEVINE2 Department of Human Genetics, The University of Michigan, Ann Arbor, Michigan 48109 of carrier DNA (salmon sperm DNA; 1 mg/ ml in TESB) were added, and the mixture ... Get Content Here

Virus-Liver Cell Interactions In Duck Hepatitis B Virus Infection
Using 0.1-0.2 yg of lz51-DHBV DNA per milliliter in the presence of 400 pgiml of sheared salmon sperm carrier DNA in 50% formamide, 6 x trisodium citrate buffer, for 40 h at 37°C. The specificity of all in situ hybridization reactions was confirmed in each experiment (7). ... Return Document

Factors Involved In Control Of Tissue-Specific Expression Of ...
Ng of sonicated salmon sperm DNA was used as carrier. Note that In lanes d, e. and m, n, binding reactions were carried out with the same amounts of proteins and carrier DNA, but slightly different concentrations of DNAase I were added. Chemical cleavages at Purina ... Retrieve Content

DNA/RNA BLOTTING & HYBRIDIZATION - Northwestern University
100 µg/ml denatured salmon sperm DNA (50% formamide if hybridizing at 42°C for RNA blots) Hybridization solution 6 x SSC 10 mM EDTA 32P labeled probe-boil with carrier DNA 5 minutes. (quick-cool) add remaining ingredients: 5x Denhardts 0.5% SDS 100 µg/ml carrier DNA Procedure: 1. Excise gel segment to be blotted. ... Fetch Doc

Lambda Library User Manual - Takarabio.com
Carrier DNA, sheared (10 µg/ml) (λ DNA or calf thymus DNA) Use of carrier DNA is optional; however, it will increase the transformation efficiency by 5- to 10-fold. Store at –20°C. Shear salmon sperm DNA by passing it 3 times through an 18-gauge needle. ... Return Document

Supplementary Materials For - Science
Biopharmaceuticals). Plasmids were introduced into yeast by the lithium acetate/salmon sperm carrier DNA/polyethylene glycol transformation method (44). Holding vectors (pCS2803, 3028, 3030, 3040, 3041, 3138, 3271-3273, 3289-3298, and 3343-3350) were constructed by amplifying the backbone, including the ... Read Document

Maurice Wilkins & DNA - YouTube
Wilkins and his team took hundreds of photos of DNA from sources including calves, mice, chickens, salmon, squid and humans. It took the best part of a decade to produce a fully verified double helix. ... View Video

GENESCREENT GENESCREEN PLUS -NR HYBRIDIZATION TRANSFER ...
0.1 mL 5 mg/mL sheared carrier DNA (e.g. herring sperm DNA, salmon sperm DNA) 107 dpm of 32P or 35S labeled probe Denature the probe solution by heating for 10 minutes at 95-100°C. Chill on ice for at least 15 minutes before adding to the prehybridization buffer. This will make the probe concentration in the hybridization solution ~ 106 dpm/mL. ... Document Viewer

Quantization Of Chloroplast DNA Using Dot Blot Filter ...
Beled ct-DNA containing carrier salmon sperm DNA were added in dot blots to nitrocellulose fil- ters (17). Application of denatured DNA at various rates using an ordinary micropipet had no reproduc- ible effect. A second nitrocellulose filter placed under the first contained only background level 151 / // ~'! 200. ... Get Document

Quantitation Of Cell-Free And Cell-Associated Kaposi’s ...
Of salmon sperm DNA as a carrier. Prior to use in PCRs, the DNA concentration of all samples was adjusted to 0.2 mg/ml. During all DNA extractions and purifications, strict precau-tions were taken to reduce the risk of false-positive results (9). Each PCR contained 2 mg of sample DNA (10 ml), 0.3 mM ... Return Doc

Kirschner Lab Buffers - Harvard University
100 ug / mL carrier DNA 400 uL 10 mg / mL salmon sperm DNA 7.2 mL DI H2O Store frozen (-20C) Hybridization mix 1 vol kinase reaction 100 vol prehybridization mix Acrylamide / Bis (30 % / 0.8 %) (200 mL) 60 g electrophoresis grade acrylamide 1.65 g bis (electrophoresis grade) DI H2O to 200 mL Stir Store at 4C wrapped in foil ... View Full Source